A piezoelectric immunosensor for early cervical cancer detection.

BACKGROUND A piezoelectric immunosensor for early cervical cancer detection was developed involving short analyis time and less invasive technique for p16INK4a, a protein that has been linked to cervical cancer. MATERIALS AND METHODS 5μL of 5.0 mg/mL p16INK4a antibody and then supernatant from different clinical samples from West China Second University Hospital (Sichuan, China) were dripped on the center of the AT-cut crystal through a micro-injector. Absorption of the p16INK4a by antibody caused a shift in the resonant frequency of the immunosensor, and the resonant frequency was correlated to the amount of the p16INK4a in the supernatant. RESULTS The greater severity of lesion grading, the greater the expression level of p16INK4. CONCLUSION Degree of cervical cancer lesion development could be determined by detected amount of p16INK4a in different clinical samples.